Hotspot

psbT-psbN

LSC · 70648-70695 · 48 bp

Back to Alopecurus

Pi 0.0625

MarkerSeek score 59.80

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 48
variable_sites: 9
pi: 0.0625
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	AAGGTTCCACCAACTCCAAC	ATCCATTCGAAGAACACGGG	50.0	50.0	57.9	58.0	110 bp	1.0	85.8
psbT-psbN_p2	TTCCGAGAACCACCTAAGGT	ATCCATTCGAAGAACACGGG	50.0	50.0	58.0	58.0	125 bp	1.0	85.4
psbT-psbN_p3	TCTTCTTCCGAGAACCACCT	ATCCATTCGAAGAACACGGG	50.0	50.0	58.0	58.0	130 bp	1.0	85.3
psbT-psbN_p4	CCACCTAAGGTTCCACCAAC	ATCCATTCGAAGAACACGGG	55.0	50.0	58.1	58.0	116 bp	1.0	85.3
psbT-psbN_p5	TCTTCTTCCGAGAACCACCT	TCGAAGAACACGGGGACTAA	50.0	50.0	58.0	58.4	124 bp	1.0	82.7