Hotspot

petA-psbJ

LSC · 60618-61464 · 847 bp

Back to Alopecurus

Pi 0.0231

MarkerSeek score 65.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 847
variable_sites: 40
pi: 0.0231
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAAAAGGTTCAGTTGTACGA	ACTGTAGCTGGTATTGCTGTG	38.1	47.6	55.4	58.0	952-962 bp	1.0	47.8
petA-psbJ_p2	ACAGTTTGAAAAGGTTCAGTTGT	ACTGTAGCTGGTATTGCTGTG	34.8	47.6	57.3	58.0	958-968 bp	1.0	46.7
petA-psbJ_p3	AGGTTCAGTTGTACGAAATGA	ACTGTAGCTGGTATTGCTGTG	38.1	47.6	55.2	58.0	947-957 bp	1.0	46.0
petA-psbJ_p4	TGAAAAGGTTCAGTTGTACGA	CTGTAGCTGGTATTGCTGTGA	38.1	47.6	55.4	57.7	951-961 bp	1.0	45.9
petA-psbJ_p5	TGAAAAGGTTCAGTTGTACGAA	ACTGTAGCTGGTATTGCTGTG	36.4	47.6	56.2	58.0	952-962 bp	1.0	45.9