Hotspot

rbcL-accD

LSC · 55647-56873 · 1227 bp

Back to Alopecurus

Pi 0.0192

MarkerSeek score 59.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1227
variable_sites: 48
pi: 0.0192
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCCTGTGTACAAGCTCGTAA	CCACCCTGATGTCCCTTCTA	50.0	55.0	57.9	58.1	1555-1566 bp	1.0	82.4
rbcL-accD_p2	AGGCTGAAACCGGTGAAATT	CCACCCTGATGTCCCTTCTA	45.0	55.0	58.0	58.1	2145-2156 bp	1.0	82.2
rbcL-accD_p3	TCTTCACATTCACCGTGCAA	CCACCCTGATGTCCCTTCTA	45.0	55.0	58.0	58.1	1961-1972 bp	1.0	82.0
rbcL-accD_p4	TTCTTCACATTCACCGTGCA	CCACCCTGATGTCCCTTCTA	45.0	55.0	58.0	58.1	1962-1973 bp	1.0	82.0
rbcL-accD_p5	AGGCTGAAACCGGTGAAATT	CTTTTCCCACCCTGATGTCC	45.0	55.0	58.0	58.2	2151-2162 bp	0.5	60.9