Hotspot

trnR-UCU-rps14

LSC · 35621-35998 · 378 bp

Back to Alopecurus

Pi 0.0185

MarkerSeek score 58.00

Primer available yes

Label trnR-UCU-rps14

Feature Metadata

label_name: trnR-UCU-rps14
feature_type: igs
region: LSC
length_bp: 378
variable_sites: 15
pi: 0.0185
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-rps14_p1	AGAAGACCTCTGTCCTATCCA	TATCCGGACACGTACTTCGA	47.6	50.0	56.9	58.0	485 bp	1.0	65.9
trnR-UCU-rps14_p2	AGGTTTAGAAGACCTCTGTCCT	TATCCGGACACGTACTTCGA	45.5	50.0	57.9	58.0	491 bp	1.0	65.2
trnR-UCU-rps14_p3	AGAAGACCTCTGTCCTATCCA	GCTATCCGGACACGTACTTC	47.6	55.0	56.9	57.9	487 bp	1.0	65.1
trnR-UCU-rps14_p4	AGAAGACCTCTGTCCTATCCA	TTACCGGGTGCAACAAGATC	47.6	50.0	56.9	58.2	444 bp	1.0	64.8
trnR-UCU-rps14_p5	AGGTTTAGAAGACCTCTGTCCT	GCTATCCGGACACGTACTTC	45.5	55.0	57.9	57.9	493 bp	1.0	64.4