Hotspot

atpI-atpH

LSC · 31369-31945 · 577 bp

Back to Alopecurus

Pi 0.0175

MarkerSeek score 57.80

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 577
variable_sites: 19
pi: 0.0175
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TTTTGCAACATTAGCCGCAG	GCAATAACAGAAGCAGCAGC	45.0	50.0	57.9	58.1	662-667 bp	1.0	81.9
atpI-atpH_p2	TTTTGCAACATTAGCCGCAG	GCAGCAATAACAGAAGCAGC	45.0	50.0	57.9	58.1	665-670 bp	1.0	81.9
atpI-atpH_p3	TTTTGCAACATTAGCCGCAG	AAGCAGCAGCAATTAGTGGA	45.0	45.0	57.9	57.8	652-657 bp	1.0	81.0
atpI-atpH_p4	TTTTGCAACATTAGCCGCAG	GCAGTACCTTGACCAACTCC	45.0	55.0	57.9	58.2	719-724 bp	1.0	80.9
atpI-atpH_p5	TTTTGCAACATTAGCCGCAG	GCCAATCCAGCAGCAATAAC	45.0	50.0	57.9	57.8	674-679 bp	1.0	80.9