primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5640	6419	868-875	1.000000	82.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5640	6419	879-886	1.000000	81.8
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	5640	6419	872-879	1.000000	81.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	0.0	16.9	0.415585	5640	6419	883-890	1.000000	80.7
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TACTCGGAGGTTCGAATCCT	20	20	45.0	50.0	58.1	57.6	8.1	16.1	0.571278	5640	6419	874-881	1.000000	79.5
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	20	20	50.0	50.0	58.1	58.0	14.6	0.0	0.109519	18210	19385	1238-1250	1.000000	83.2
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	20	20	50.0	50.0	58.1	57.8	14.6	0.0	0.250044	18210	19385	1245-1257	1.000000	82.1
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	18210	19385	1253-1265	1.000000	80.9
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	20	20	50.0	55.0	58.1	58.3	14.6	0.0	0.395580	18210	19385	1252-1264	1.000000	80.9
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	AAAGGATTTGCAGTCCCCTG	GGACATTCCCTCGTTTCCAT	20	20	50.0	50.0	58.1	57.6	14.6	0.0	0.491190	18210	19385	1242-1254	1.000000	80.2
atpI-atpH_p1	atpI-atpH	1	TTTTGCAACATTAGCCGCAG	GCAATAACAGAAGCAGCAGC	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.157706	31369	31945	662-667	1.000000	81.9
atpI-atpH_p2	atpI-atpH	2	TTTTGCAACATTAGCCGCAG	GCAGCAATAACAGAAGCAGC	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.157706	31369	31945	665-670	1.000000	81.9
atpI-atpH_p3	atpI-atpH	3	TTTTGCAACATTAGCCGCAG	AAGCAGCAGCAATTAGTGGA	20	20	45.0	45.0	57.9	57.8	0.0	3.2	0.268178	31369	31945	652-657	1.000000	81.0
atpI-atpH_p4	atpI-atpH	4	TTTTGCAACATTAGCCGCAG	GCAGTACCTTGACCAACTCC	20	20	45.0	55.0	57.9	58.2	0.0	0.0	0.253472	31369	31945	719-724	1.000000	80.9
atpI-atpH_p5	atpI-atpH	5	TTTTGCAACATTAGCCGCAG	GCCAATCCAGCAGCAATAAC	20	20	45.0	50.0	57.9	57.8	0.0	0.0	0.277543	31369	31945	674-679	1.000000	80.9
trnR-UCU-rps14_p1	trnR-UCU-rps14	1	AGAAGACCTCTGTCCTATCCA	TATCCGGACACGTACTTCGA	21	20	47.6	50.0	56.9	58.0	0.0	6.1	2.074369	35621	35998	485-485	1.000000	65.9
trnR-UCU-rps14_p2	trnR-UCU-rps14	2	AGGTTTAGAAGACCTCTGTCCT	TATCCGGACACGTACTTCGA	22	20	45.5	50.0	57.9	58.0	0.0	6.1	2.157697	35621	35998	491-491	1.000000	65.2
trnR-UCU-rps14_p3	trnR-UCU-rps14	3	AGAAGACCTCTGTCCTATCCA	GCTATCCGGACACGTACTTC	21	20	47.6	55.0	56.9	57.9	0.0	6.1	2.168392	35621	35998	487-487	1.000000	65.1
trnR-UCU-rps14_p4	trnR-UCU-rps14	4	AGAAGACCTCTGTCCTATCCA	TTACCGGGTGCAACAAGATC	21	20	47.6	50.0	56.9	58.2	0.0	10.7	2.235530	35621	35998	444-444	1.000000	64.8
trnR-UCU-rps14_p5	trnR-UCU-rps14	5	AGGTTTAGAAGACCTCTGTCCT	GCTATCCGGACACGTACTTC	22	20	45.5	55.0	57.9	57.9	0.0	6.1	2.251720	35621	35998	493-493	1.000000	64.4
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.212764	43608	44229	794-805	1.000000	81.2
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTCAA	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.254079	43608	44229	744-755	1.000000	81.1
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TCTCCTGAGGTTGTCGGAAT	AGTTCCAATGCTACGCCTTC	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.322349	43608	44229	746-757	1.000000	80.5
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTCTCCTGAGGTTGTCGGAA	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.439292	43608	44229	795-806	1.000000	79.4
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTTCTCCTGAGGTTGTCGGA	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.439292	43608	44229	796-807	1.000000	79.4
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	45551	46379	915-943	1.000000	83.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	45551	46379	907-935	1.000000	82.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	45551	46379	885-913	1.000000	82.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	45551	46379	905-933	1.000000	81.3
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	45551	46379	914-942	1.000000	81.0
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	CGCGCCTTAAACGTATCCTA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.175907	47370	47923	681-698	1.000000	82.3
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	20	20	50.0	55.0	58.1	58.2	0.0	0.0	0.287928	47370	47923	655-672	1.000000	81.6
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	ATGATAATCATCCGCGCCTT	20	20	50.0	45.0	58.1	57.5	0.0	0.0	0.531462	47370	47923	693-710	1.000000	79.4
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGGTGGC	20	20	50.0	50.0	58.1	57.5	0.0	0.0	0.559303	47370	47923	656-673	1.000000	79.4
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	ATCCGCGCCTTAAACGTATC	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.557071	47370	47923	684-701	1.000000	79.3
rbcL-accD_p1	rbcL-accD	1	GCCTGTGTACAAGCTCGTAA	CCACCCTGATGTCCCTTCTA	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.194972	55647	56873	1555-1566	1.000000	82.4
rbcL-accD_p2	rbcL-accD	2	AGGCTGAAACCGGTGAAATT	CCACCCTGATGTCCCTTCTA	20	20	45.0	55.0	58.0	58.1	8.5	0.0	0.125049	55647	56873	2145-2156	1.000000	82.2
rbcL-accD_p3	rbcL-accD	3	TCTTCACATTCACCGTGCAA	CCACCCTGATGTCCCTTCTA	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.160008	55647	56873	1961-1972	1.000000	82.0
rbcL-accD_p4	rbcL-accD	4	TTCTTCACATTCACCGTGCA	CCACCCTGATGTCCCTTCTA	20	20	45.0	55.0	58.0	58.1	0.0	0.0	0.160008	55647	56873	1962-1973	1.000000	82.0
rbcL-accD_p5	rbcL-accD	5	AGGCTGAAACCGGTGAAATT	CTTTTCCCACCCTGATGTCC	20	20	45.0	55.0	58.0	58.2	8.5	0.0	0.165200	55647	56873	2151-2162	0.500000	60.9
petA-psbJ_p1	petA-psbJ	1	TGAAAAGGTTCAGTTGTACGA	ACTGTAGCTGGTATTGCTGTG	21	21	38.1	47.6	55.4	58.0	0.0	0.0	4.610874	60618	61464	952-962	1.000000	47.8
petA-psbJ_p2	petA-psbJ	2	ACAGTTTGAAAAGGTTCAGTTGT	ACTGTAGCTGGTATTGCTGTG	23	21	34.8	47.6	57.3	58.0	0.0	0.0	4.744107	60618	61464	958-968	1.000000	46.7
petA-psbJ_p3	petA-psbJ	3	AGGTTCAGTTGTACGAAATGA	ACTGTAGCTGGTATTGCTGTG	21	21	38.1	47.6	55.2	58.0	0.0	0.0	4.834691	60618	61464	947-957	1.000000	46.0
petA-psbJ_p4	petA-psbJ	4	TGAAAAGGTTCAGTTGTACGA	CTGTAGCTGGTATTGCTGTGA	21	21	38.1	47.6	55.4	57.7	0.0	0.0	4.840266	60618	61464	951-961	1.000000	45.9
petA-psbJ_p5	petA-psbJ	5	TGAAAAGGTTCAGTTGTACGAA	ACTGTAGCTGGTATTGCTGTG	22	21	36.4	47.6	56.2	58.0	0.0	0.0	4.851353	60618	61464	952-962	1.000000	45.9
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	GCCAATAAATAGAGCTGGGGT	20	21	45.0	47.6	58.0	57.8	0.0	0.0	1.247037	62234	63513	1207-1476	1.000000	75.8
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	GCCAATAAATAGAGCTGGGGT	20	21	50.0	47.6	57.9	57.8	6.4	0.0	1.281031	62234	63513	1196-1465	1.000000	75.6
psbE-petL_p3	psbE-petL	3	ACCAACCCGCAATGAATAGG	GCCAATAAATAGAGCTGGGGT	20	21	50.0	47.6	58.2	57.8	0.0	0.0	1.466989	62234	63513	1198-1467	1.000000	74.1
psbE-petL_p4	psbE-petL	4	TGACAAATAACCAACCCGCA	AGCCAATAAATAGAGCTGGGG	20	21	45.0	47.6	58.0	57.5	0.0	0.0	1.532941	62234	63513	1208-1477	1.000000	73.5
psbE-petL_p5	psbE-petL	5	CAACCCGCAATGAATAGGGA	AGCCAATAAATAGAGCTGGGG	20	21	50.0	47.6	57.9	57.5	6.4	0.0	1.566936	62234	63513	1197-1466	1.000000	73.3
psbT-psbN_p1	psbT-psbN	1	AAGGTTCCACCAACTCCAAC	ATCCATTCGAAGAACACGGG	20	20	50.0	50.0	57.9	58.0	0.0	3.3	0.091541	70648	70695	110-110	1.000000	85.8
psbT-psbN_p2	psbT-psbN	2	TTCCGAGAACCACCTAAGGT	ATCCATTCGAAGAACACGGG	20	20	50.0	50.0	58.0	58.0	0.0	3.3	0.043983	70648	70695	125-125	1.000000	85.4
psbT-psbN_p3	psbT-psbN	3	TCTTCTTCCGAGAACCACCT	ATCCATTCGAAGAACACGGG	20	20	50.0	50.0	58.0	58.0	0.0	3.3	0.035298	70648	70695	130-130	1.000000	85.3
psbT-psbN_p4	psbT-psbN	4	CCACCTAAGGTTCCACCAAC	ATCCATTCGAAGAACACGGG	20	20	55.0	50.0	58.1	58.0	0.0	3.3	0.118538	70648	70695	116-116	1.000000	85.3
psbT-psbN_p5	psbT-psbN	5	TCTTCTTCCGAGAACCACCT	TCGAAGAACACGGGGACTAA	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.391468	70648	70695	124-124	1.000000	82.7
rpl22-rps19_p1	rpl22-rps19	1	AGATGGGATAAGACGCTCGA	CAAATTGGGGGAATTCGTGC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026092	80414	80491	404-404	1.000000	82.8
rpl22-rps19_p2	rpl22-rps19	2	AGATGGGATAAGACGCTCGA	GCAAATTGGGGGAATTCGTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.026092	80414	80491	405-405	1.000000	82.8
rpl22-rps19_p3	rpl22-rps19	3	GCCAGCGAATCTCATCAAGA	CAAATTGGGGGAATTCGTGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.063364	80414	80491	341-341	1.000000	82.8
rpl22-rps19_p4	rpl22-rps19	4	AGATGGGATAAGACGCTCGA	GGTTGGCCATACAATAGCGA	20	20	50.0	50.0	58.0	58.0	0.0	6.1	0.055546	80414	80491	479-479	1.000000	82.1
rpl22-rps19_p5	rpl22-rps19	5	AGATGGGATAAGACGCTCGA	GTTATCCTGCGCTTGGAAGA	20	20	50.0	50.0	58.0	58.0	0.0	22.4	0.041515	80414	80491	946-949	1.000000	81.4
ndhF-rpl32_p1	ndhF-rpl32	1	GGAAGTGGGAGAAGAGGGAT	TGCTAAAGAATAAGCGTGTACT	20	22	55.0	36.4	58.1	55.6	0.0	0.0	4.531268	104361	105259	1028-1055	1.000000	48.7
ndhF-rpl32_p2	ndhF-rpl32	2	GGCCAAACCCCATTGACATA	TGCTAAAGAATAAGCGTGTACT	20	22	50.0	36.4	58.1	55.6	0.0	0.0	4.558388	104361	105259	1054-1081	1.000000	48.4
ndhF-rpl32_p3	ndhF-rpl32	3	AAAAGGCCAAACCCCATTGA	TGCTAAAGAATAAGCGTGTACT	20	22	45.0	36.4	58.2	55.6	5.0	0.0	4.604588	104361	105259	1058-1085	1.000000	48.0
ndhF-rpl32_p4	ndhF-rpl32	4	GGAAGTGGGAGAAGAGGGAT	TTGCTAAAGAATAAGCGTGTACT	20	23	55.0	34.8	58.1	56.3	0.0	0.0	4.811693	104361	105259	1029-1056	1.000000	46.5
ndhF-rpl32_p5	ndhF-rpl32	5	GGCCAAACCCCATTGACATA	TTGCTAAAGAATAAGCGTGTACT	20	23	50.0	34.8	58.1	56.3	0.0	0.0	4.838814	104361	105259	1055-1082	1.000000	46.1
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.130936	105440	106186	846-918	1.000000	85.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.130936	105440	106186	845-917	1.000000	85.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGGTTTTTCTGGGCAACAA	ACTGAGATTGGAGAGCAGGT	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.177428	105440	106186	1339-1411	1.000000	83.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AAGGGTTTTTCTGGGCAACA	GATCCGCACAAAAGAGTTGC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.158124	105440	106186	1398-1470	1.000000	83.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGTTTTTCTGGGCAACAA	GATCCGCACAAAAGAGTTGC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.158124	105440	106186	1397-1469	1.000000	83.1
ndhG-ndhI_p1	ndhG-ndhI	1	TCTCATGTATTGGTCCAGGT	TCGAATTCAAGAACGATTACTGA	20	23	45.0	34.8	55.5	56.1	0.0	6.2	7.402104	110838	111088	303-319	1.000000	46.7
ndhG-ndhI_p2	ndhG-ndhI	2	TCTCATGTATTGGTCCAGGT	TTCGAATTCAAGAACGATTACTGA	20	24	45.0	33.3	55.5	56.7	0.0	6.2	7.735707	110838	111088	304-320	1.000000	46.7
ndhG-ndhI_p3	ndhG-ndhI	3	TCTCATGTATTGGTCCAGGT	CTTCGAATTCAAGAACGATTACTGA	20	25	45.0	36.0	55.5	57.7	0.0	6.2	7.753507	110838	111088	305-321	1.000000	46.7
ndhG-ndhI_p4	ndhG-ndhI	4	TCTCATGTATTGGTCCAGGT	TCTTCGAATTCAAGAACGATTACTG	20	25	45.0	36.0	55.5	57.7	0.0	14.2	7.753507	110838	111088	306-322	1.000000	46.7
ndhG-ndhI_p5	ndhG-ndhI	5	TCTCATGTATTGGTCCAGGT	TCTTCGAATTCAAGAACGAT	20	20	45.0	35.0	55.5	52.7	0.0	14.2	7.789194	110838	111088	306-322	1.000000	46.7