Hotspot

ycf1

SSC · 121610-127009 · 5400 bp

Pi 0.0133

MarkerSeek score 48.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5400
variable_sites: 106
pi: 0.0133
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGTATGATCCACGGTTTCA	GCCCGGACCAATATGCATAT	45.0	50.0	56.5	57.9	261-276 bp	1.0	70.9
ycf1_p2	TGGTATGATCCACGGTTTCA	TATTGAGGCCCGGACCAATA	45.0	50.0	56.5	58.2	268-283 bp	1.0	70.3
ycf1_p3	TGGTATGATCCACGGTTTCA	TATCGATATTGAGGCCCGGA	45.0	50.0	56.5	57.8	274-289 bp	1.0	69.9
ycf1_p4	TGATTGGTATGATCCACGGT	GCCCGGACCAATATGCATAT	45.0	50.0	56.3	57.9	265-280 bp	1.0	69.0
ycf1_p5	TTGGTATGATCCACGGTTTCA	GCCCGGACCAATATGCATAT	42.9	50.0	57.3	57.9	262-277 bp	1.0	68.9