Hotspot

petD

LSC · 75603-76830 · 1228 bp

Back to Aloe

Pi 0.00896
MarkerSeek score 51.20
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1228
variable_sites
16
pi
0.00896
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 TCTCCGAAGAGAGGATGGAT TGGTATTTCACAGTTGAATCA 50.0 33.3 56.9 53.1 1272-1279 bp 1.0 41.1
petD_p2 TCTCCGAAGAGAGGATGGAT TGGTATTTCACAGTTGAATCAA 50.0 31.8 56.9 53.9 1272-1279 bp 1.0 41.1
petD_p3 TCTCCGAAGAGAGGATGGATT TGGTATTTCACAGTTGAATCA 47.6 33.3 57.7 53.1 1272-1279 bp 1.0 41.1
petD_p4 TCTCCGAAGAGAGGATGGATT TGGTATTTCACAGTTGAATCAA 47.6 31.8 57.7 53.9 1272-1279 bp 1.0 41.1
petD_p5 TCTCCGAAGAGAGGATGGAT TGGTATTTCACAGTTGAATCAAT 50.0 30.4 56.9 54.5 1272-1279 bp 1.0 41.1