Hotspot

ndhF

SSC · 109626-111845 · 2220 bp

Back to Aloe

Pi 0.0108
MarkerSeek score 51.20
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2220
variable_sites
35
pi
0.0108
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACAGGAACAAGAGGGATCCA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 2832-2862 bp 0.666667 67.1
ndhF_p2 ACAGGAACAAGAGGGATCCA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 2833-2863 bp 0.666667 67.1
ndhF_p3 AAGAGGGATCCACCGAAGAA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.0 58.0 2824-2854 bp 0.666667 67.1
ndhF_p4 AAGAGGGATCCACCGAAGAA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.0 58.0 2825-2855 bp 0.666667 67.1
ndhF_p5 ACAGGAACAAGAGGGATCCA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 2842-2872 bp 0.666667 66.2