Hotspot

trnN-GUU-trnR-ACG

IRa · 127439-128034 · 596 bp

Back to Aloe

Pi 0.00559
MarkerSeek score 62.20
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
596
variable_sites
5
pi
0.00559
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.0 57.8 737 bp 1.0 78.8
trnN-GUU-trnR-ACG_p2 GCTGTTAACTGACTGGTCGT CAGAGGATTAGAGCACGTGG 50.0 55.0 57.8 57.8 701 bp 1.0 77.6
trnN-GUU-trnR-ACG_p3 TGGTCGTAGGTTCGAATCCT CAGAGGATTAGAGCACGTGG 50.0 55.0 58.2 57.8 688 bp 1.0 77.6
trnN-GUU-trnR-ACG_p4 CTGGTCGTAGGTTCGAATCC CAGAGGATTAGAGCACGTGG 55.0 55.0 57.8 57.8 689 bp 1.0 77.2
trnN-GUU-trnR-ACG_p5 TCTCCCGTTGTTCCTCAGTA CAGAGGATTAGAGCACGTGG 50.0 55.0 57.7 57.8 741 bp 1.0 76.4