Hotspot

rps15-ycf1

SSC · 121261-121609 · 349 bp

Back to Aloe

Pi 0.0253
MarkerSeek score 61.30
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
349
variable_sites
13
pi
0.0253
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TGAAATTCAACAGATCCGCA GCAGTCATTTCAGTATGTCAAGA 40.0 39.1 55.7 56.9 472-476 bp 1.0 43.7
rps15-ycf1_p2 CTTGAAATTCAACAGATCCGCA GCAGTCATTTCAGTATGTCAAGA 40.9 39.1 57.6 56.9 474-478 bp 1.0 43.7
rps15-ycf1_p3 ACTTGAAATTCAACAGATCCGC GCAGTCATTTCAGTATGTCAAGA 40.9 39.1 57.6 56.9 475-479 bp 1.0 43.7
rps15-ycf1_p4 TGAAATTCAACAGATCCGCAA GCAGTCATTTCAGTATGTCAAGA 38.1 39.1 56.4 56.9 472-476 bp 1.0 43.7
rps15-ycf1_p5 TTGAAATTCAACAGATCCGCA GCAGTCATTTCAGTATGTCAAGA 38.1 39.1 56.4 56.9 473-477 bp 1.0 43.7