Hotspot

psaC-ndhE

SSC · 115205-115565 · 361 bp

Back to Aloe

Pi 0.024
MarkerSeek score 69.10
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
361
variable_sites
13
pi
0.024
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 ACATCCGTCCCAAGGTATCA GGAAGCAGCTATTGGGCTAG 50.0 55.0 58.1 58.1 554-556 bp 1.0 80.0
psaC-ndhE_p2 CAATACATCTGTGGGGCAGG GGAAGCAGCTATTGGGCTAG 55.0 55.0 58.3 58.1 530-532 bp 1.0 78.5
psaC-ndhE_p3 CCAATACATCTGTGGGGCAG GGAAGCAGCTATTGGGCTAG 55.0 55.0 58.3 58.1 531-533 bp 1.0 78.5
psaC-ndhE_p4 CAGGCTCGTACACATTGAGT GGAAGCAGCTATTGGGCTAG 50.0 55.0 57.6 58.1 514-516 bp 1.0 78.2
psaC-ndhE_p5 AGCTTTACATCCGTCCCAAG GGAAGCAGCTATTGGGCTAG 50.0 55.0 57.6 58.1 560-562 bp 1.0 77.7