Hotspot

rps11-rpl36

LSC · 78534-78662 · 129 bp

Back to Aloe

Pi 0.0312
MarkerSeek score 70.10
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
129
variable_sites
5
pi
0.0312
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 AATACGTCCATTCCTACGCG TTGTGAAAAGTGCCGACTGA 50.0 45.0 57.9 58.0 258-262 bp 1.0 81.5
rps11-rpl36_p2 AATACGTCCATTCCTACGCG TGAAAAGTGCCGACTGATCC 50.0 50.0 57.9 58.2 255-259 bp 1.0 80.2
rps11-rpl36_p3 AATACGTCCATTCCTACGCG TGTGAAAAGTGCCGACTGAT 50.0 45.0 57.9 57.7 257-261 bp 1.0 79.7
rps11-rpl36_p4 AATACGTCCATTCCTACGCG AAGGGCTTCGGTTCGTAAAA 50.0 45.0 57.9 57.7 279-283 bp 1.0 79.4
rps11-rpl36_p5 AATACGTCCATTCCTACGCG AGGGCTTCGGTTCGTAAAAT 50.0 45.0 57.9 57.5 278-282 bp 1.0 77.7