Hotspot

petD-rpoA

LSC · 76831-77025 · 195 bp

Back to Aloe

Pi 0.0547
MarkerSeek score 71.10
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
195
variable_sites
16
pi
0.0547
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 TTGATTGGTACCGCAGTAGC TGCTGTATCCATGCCTGTTC 50.0 50.0 58.0 58.0 790 bp 1.0 82.0
petD-rpoA_p2 AAAACCCATTTCGTCGTCCA TGCTGTATCCATGCCTGTTC 45.0 50.0 58.0 58.0 828 bp 1.0 81.9
petD-rpoA_p3 TCCAAAACCCATTTCGTCGT TGCTGTATCCATGCCTGTTC 45.0 50.0 58.0 58.0 831 bp 1.0 81.9
petD-rpoA_p4 TTGCAACTCCGGAAATGGAA TGCTGTATCCATGCCTGTTC 45.0 50.0 58.0 58.0 1385-1393 bp 1.0 81.4
petD-rpoA_p5 TTGCAACTCCGGAAATGGAA TAGCTAACCTGACGGAACCA 45.0 50.0 58.0 58.1 1497-1505 bp 1.0 81.1