Hotspot

petA-psbJ

LSC · 61848-62975 · 1128 bp

Back to Aloe

Pi 0.0297
MarkerSeek score 67.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1128
variable_sites
50
pi
0.0297
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCATCCGTTATTTTGGCAC ATTCCGGGTTAGGGTCATCT 50.0 50.0 58.1 57.8 1236-1243 bp 1.0 81.5
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC ATTCCGGGTTAGGGTCATCT 50.0 50.0 57.8 57.8 1253-1260 bp 1.0 80.9
petA-psbJ_p3 GGCATCCGTTATTTTGGCAC CCGGGTTAGGGTCATCTCTA 50.0 55.0 58.1 57.6 1233-1240 bp 1.0 80.0
petA-psbJ_p4 TGTTCTTCTTGGCATCCGTT ATTCCGGGTTAGGGTCATCT 45.0 50.0 57.7 57.8 1246-1253 bp 1.0 80.0
petA-psbJ_p5 TTGTTCTTCTTGGCATCCGT ATTCCGGGTTAGGGTCATCT 45.0 50.0 57.7 57.8 1247-1254 bp 1.0 79.9