Hotspot

ndhC-trnV-UAC

LSC · 49597-50305 · 709 bp

Back to Aloe

Pi 0.0217
MarkerSeek score 64.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
709
variable_sites
23
pi
0.0217
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAGATAGGAATAACACCC AGAAGGTCTACGGTTCGAGT 45.5 50.0 57.8 57.8 814-819 bp 1.0 63.5
ndhC-trnV-UAC_p2 TGCCAAGATAGGAATAACACCC AGCCCAATGCAACAAAATGC 45.5 45.0 57.8 58.5 896-901 bp 1.0 61.1
ndhC-trnV-UAC_p3 TGCCAAGATAGGAATAACACCC GAAGGTCTACGGTTCGAGTC 45.5 55.0 57.8 57.5 813-818 bp 1.0 60.8
ndhC-trnV-UAC_p4 TGCCAAGATAGGAATAACACCC GTTCGAGTCCGTATAGCCCT 45.5 55.0 57.8 58.7 802-807 bp 1.0 59.7
ndhC-trnV-UAC_p5 CCAAGATAGGAATAACACCCGA AGAAGGTCTACGGTTCGAGT 45.5 50.0 57.3 57.8 812-817 bp 1.0 59.4