Hotspot

trnT-GGU-psbD

LSC · 30830-31813 · 984 bp

Back to Aloe

Pi 0.0149
MarkerSeek score 59.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
984
variable_sites
22
pi
0.0149
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG CCCTCCGTAACCAATCATCC 55.0 55.0 58.1 57.7 1118-1122 bp 1.0 79.0
trnT-GGU-psbD_p2 TGAATTGAATAGGGCAGGGC CCCTCCGTAACCAATCATCC 50.0 55.0 57.9 57.7 1150-1154 bp 1.0 78.9
trnT-GGU-psbD_p3 TCAGTGGTAGAGTAACGCCA CCCTCCGTAACCAATCATCC 50.0 55.0 58.1 57.7 1121-1125 bp 1.0 78.8
trnT-GGU-psbD_p4 GGCGTAAGTCATCGGTTCAA CCCTCCGTAACCAATCATCC 50.0 55.0 58.3 57.7 1095-1099 bp 1.0 77.3
trnT-GGU-psbD_p5 GGCCCTTTTAACTCAGTGGT CCCTCCGTAACCAATCATCC 50.0 55.0 57.7 57.7 1133-1137 bp 1.0 77.2