Hotspot

trnE-UUC-trnT-GGU

LSC · 30463-30757 · 295 bp

Back to Aloe

Pi 0.0295
MarkerSeek score 64.60
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
295
variable_sites
13
pi
0.0295
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 375-390 bp 1.0 81.7
trnE-UUC-trnT-GGU_p2 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 396-411 bp 1.0 81.6
trnE-UUC-trnT-GGU_p3 TGTCCTGAACCACTAGACGA CCATGGCGTTACTCTACCAC 50.0 55.0 57.7 58.1 354-369 bp 1.0 81.5
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG CCATGGCGTTACTCTACCAC 55.0 55.0 58.3 58.1 375-390 bp 1.0 81.4
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG TGGCGTTACTCTACCACTGA 55.0 50.0 58.3 58.1 372-387 bp 1.0 81.2