Hotspot

psbK-psbI

LSC · 7485-7898 · 414 bp

Back to Aloe

Pi 0.022
MarkerSeek score 66.20
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
414
variable_sites
12
pi
0.022
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG GGCCCGGATCGTTAGATAAG 50.0 55.0 57.9 57.6 498-532 bp 1.0 82.5
psbK-psbI_p2 ATGCCCGTCATACCTGTACT GGCCCGGATCGTTAGATAAG 50.0 55.0 58.2 57.6 537-571 bp 1.0 81.1
psbK-psbI_p3 TGGCAAGCTGCTGTAAGTTT GGCCCGGATCGTTAGATAAG 45.0 55.0 58.3 57.6 495-529 bp 1.0 80.5
psbK-psbI_p4 TTGGCAAGCTGCTGTAAGTT GGCCCGGATCGTTAGATAAG 45.0 55.0 58.3 57.6 496-530 bp 1.0 80.5
psbK-psbI_p5 TTTGGCAAGCTGCTGTAAGT GGCCCGGATCGTTAGATAAG 45.0 55.0 58.3 57.6 497-531 bp 1.0 80.5