Hotspot

rps16-trnQ-UUG

LSC · 5811-6881 · 1071 bp

Back to Aloe

Pi 0.0206
MarkerSeek score 62.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1071
variable_sites
33
pi
0.0206
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1151-1169 bp 1.0 82.4
rps16-trnQ-UUG_p2 GTCTTTCAAGTCGCACGTTG GTCACTCGGAGGTTCGAATC 50.0 55.0 58.0 58.1 1159-1177 bp 1.0 82.3
rps16-trnQ-UUG_p3 ATGTCTTTCAAGTCGCACGT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1153-1171 bp 1.0 81.3
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1144-1162 bp 1.0 81.3
rps16-trnQ-UUG_p5 GTCTTTCAAGTCGCACGTTG CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.0 57.8 1155-1173 bp 1.0 81.3