Hotspot

trnK-UUU-rps16

LSC · 4023-4683 · 661 bp

Back to Aloe

Pi 0.0265
MarkerSeek score 66.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
661
variable_sites
25
pi
0.0265
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TGAGGAAGGCAGAATTATTGA 50.0 38.1 58.2 54.6 736-739 bp 1.0 47.9
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TGAGGAAGGCAGAATTATTGA 50.0 38.1 58.2 54.6 735-738 bp 1.0 47.9
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TGAGGAAGGCAGAATTATTGAA 50.0 36.4 58.2 55.4 736-739 bp 1.0 46.4
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TGAGGAAGGCAGAATTATTGAA 50.0 36.4 58.2 55.4 735-738 bp 1.0 46.4
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTGAGGAAGGCAGAATTATTGA 50.0 36.4 58.2 55.4 736-739 bp 1.0 46.4