Hotspot

rpl16

LSC · 86949-88817 · 1869 bp

Back to Alocasia

Pi 0.12

MarkerSeek score 82.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1869
variable_sites: 201
pi: 0.12
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ACACATCAATTCTCGAGCCC	TTCCCCTACAAACCATTCGC	50.0	50.0	58.0	58.2	2181-2323 bp	1.0	90.0
rpl16_p2	ATTGGGCACTGCTTCTTTGA	TTCCCCTACAAACCATTCGC	45.0	50.0	58.0	58.2	2407 bp	0.5	58.6
rpl16_p3	TGGGCACTGCTTCTTTGATT	TTCCCCTACAAACCATTCGC	45.0	50.0	58.0	58.2	2405 bp	0.5	58.6
rpl16_p4	TTGGGCACTGCTTCTTTGAT	TTCCCCTACAAACCATTCGC	45.0	50.0	58.0	58.2	2406 bp	0.5	58.6
rpl16_p5	TCTCAATTCCCTGGCGATTG	TTCCCCTACAAACCATTCGC	50.0	50.0	58.0	58.2	2581 bp	0.5	58.3