Hotspot

ndhB-trnL-CAA

IRa · 153103-154376 · 1274 bp

Back to Alocasia

Pi 0.0428

MarkerSeek score 66.40

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 1274
variable_sites: 35
pi: 0.0428
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.64
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	899-1334 bp	1.0	86.0
ndhB-trnL-CAA_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	897-1332 bp	1.0	86.0
ndhB-trnL-CAA_p3	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	894-1329 bp	1.0	84.5
ndhB-trnL-CAA_p4	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	898-1333 bp	1.0	83.9
ndhB-trnL-CAA_p5	TGCAATTGCTCAGGATACCC	TCGCGTGTCTACCATTTCAC	50.0	50.0	57.9	58.3	896-1331 bp	1.0	83.7