Hotspot

rps15-ycf1

SSC · 131831-132673 · 843 bp

Back to Alocasia

Pi 0.188

MarkerSeek score 83.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 843
variable_sites: 151
pi: 0.188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AACACCATGCATTGATGGGT	TCATTGAACCGGTTCGTCTC	45.0	50.0	58.1	57.9	1877-2114 bp	1.0	87.9
rps15-ycf1_p2	AACACCATGCATTGATGGGT	CATTGAACCGGTTCGTCTCT	45.0	50.0	58.1	57.9	1876-2113 bp	1.0	87.9
rps15-ycf1_p3	TCCCAACGCGTTACATAAGG	TCATTGAACCGGTTCGTCTC	50.0	50.0	58.0	57.9	2014-2251 bp	1.0	87.8
rps15-ycf1_p4	TCCCAACGCGTTACATAAGG	CATTGAACCGGTTCGTCTCT	50.0	50.0	58.0	57.9	2013-2250 bp	1.0	87.8
rps15-ycf1_p5	TTCCCAATTGTTCTGGTGCA	TCATTGAACCGGTTCGTCTC	45.0	50.0	57.9	57.9	2073-2310 bp	1.0	87.1