Hotspot

ndhG-ndhI

SSC · 127020-127415 · 396 bp

Back to Alocasia

Pi 0.123
MarkerSeek score 80.90
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
396
variable_sites
46
pi
0.123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 TGTATTGGCCCAGTTAAATCCA TTCAAGGACAGTTACCAATT 40.9 35.0 57.7 52.3 441-540 bp 1.0 51.1
ndhG-ndhI_p2 TGTATTGGCCCAGTTAAATCCA ATTCAAGGACAGTTACCAAT 40.9 35.0 57.7 52.0 442-541 bp 1.0 51.1
ndhG-ndhI_p3 TGTATTGGCCCAGTTAAATCCA ATTCAAGGACAGTTACCAATT 40.9 33.3 57.7 53.0 442-541 bp 1.0 51.1
ndhG-ndhI_p4 TGTATTGGCCCAGTTAAATCCA TCAAGGACAGTTACCAATTAG 40.9 38.1 57.7 52.9 440-539 bp 1.0 51.1
ndhG-ndhI_p5 TGTATTGGCCCAGTTAAATCCA TTCAAGGACAGTTACCAATTAG 40.9 36.4 57.7 53.8 441-540 bp 1.0 51.1