Hotspot

psaC-ndhE

SSC · 124950-125785 · 836 bp

Back to Alocasia

Pi 0.0987

MarkerSeek score 83.20

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 836
variable_sites: 70
pi: 0.0987
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GGCCCGTACACATTGAGTAC	CGTAACAGAAAATCAACGCGT	55.0	42.9	58.4	57.8	947-1089 bp	1.0	77.4
psaC-ndhE_p2	GCCCGTACACATTGAGTACA	CGTAACAGAAAATCAACGCGT	50.0	42.9	57.6	57.8	946-1088 bp	1.0	77.2
psaC-ndhE_p3	AGGCCCGTACACATTGAGTA	CGTAACAGAAAATCAACGCGT	50.0	42.9	58.4	57.8	948-1090 bp	1.0	76.7
psaC-ndhE_p4	GGCCCGTACACATTGAGTAC	TCGTAACAGAAAATCAACGCG	55.0	42.9	58.4	57.6	948-1090 bp	1.0	75.3
psaC-ndhE_p5	GCCCGTACACATTGAGTACA	TCGTAACAGAAAATCAACGCG	50.0	42.9	57.6	57.6	947-1089 bp	1.0	75.1