Hotspot

trnL-CAA-ndhB

IRb · 100611-101884 · 1274 bp

Back to Alocasia

Pi 0.0465

MarkerSeek score 68.40

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 1274
variable_sites: 38
pi: 0.0465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.64
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	898-1334 bp	1.0	86.2
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.0	57.9	896-1332 bp	1.0	86.2
trnL-CAA-ndhB_p3	GCGTGTCTACCATTTCACCA	TGCAATTGCTCAGGATACCC	50.0	50.0	58.2	57.9	893-1329 bp	1.0	84.7
trnL-CAA-ndhB_p4	TCTCGCGTGTCTACCATTTC	TGCAATTGCTCAGGATACCC	50.0	50.0	57.7	57.9	897-1333 bp	1.0	84.1
trnL-CAA-ndhB_p5	TCGCGTGTCTACCATTTCAC	TGCAATTGCTCAGGATACCC	50.0	50.0	58.3	57.9	895-1331 bp	1.0	83.9