Hotspot

rps4-trnT-UGU

LSC · 49518-50020 · 503 bp

Back to Alocasia

Pi 0.12

MarkerSeek score 83.00

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 503
variable_sites: 59
pi: 0.12
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CCCCAGACGGCGTATTTTT	GATGGTCATCGGTTCGACTC	52.6	55.0	58.1	58.2	583-741 bp	1.0	78.2
rps4-trnT-UGU_p2	CCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	52.6	55.0	58.1	57.9	614-772 bp	1.0	78.0
rps4-trnT-UGU_p3	CCCCAGACGGCGTATTTTT	CCGCTTAGCTCAGAGGTTAG	52.6	55.0	58.1	57.5	622-780 bp	1.0	75.0
rps4-trnT-UGU_p4	CCCCAGACGGCGTATTTTT	CAGAGGTTAGAGCATCGCAT	52.6	50.0	58.1	57.5	612-770 bp	1.0	74.9
rps4-trnT-UGU_p5	CCCCAGACGGCGTATTTTT	TCAGAGGTTAGAGCATCGCA	52.6	50.0	58.1	58.5	613-771 bp	1.0	74.9