Hotspot

trnK-UUU-rps16

LSC · 4547-5350 · 804 bp

Back to Alocasia

Pi 0.0947

MarkerSeek score 80.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 68
pi: 0.0947
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCAGGATCAGTCGTGG	AGCTCAACCCACAAGAACTG	55.0	50.0	58.0	58.0	951-991 bp	1.0	89.5
trnK-UUU-rps16_p2	CCTTTCAGGATCAGTCGTGG	AGAGATCTTCGGAGAGTGGG	55.0	55.0	58.0	57.9	1040-1080 bp	1.0	89.1
trnK-UUU-rps16_p3	TCAGGATCAGTCGTGGTCTT	AGCTCAACCCACAAGAACTG	50.0	50.0	58.1	58.0	947-987 bp	1.0	89.1
trnK-UUU-rps16_p4	CCTTTCAGGATCAGTCGTGG	TTCTTTCTTGCTTGAGCCGT	55.0	45.0	58.0	58.0	1166-1211 bp	1.0	88.9
trnK-UUU-rps16_p5	CCTTTCAGGATCAGTCGTGG	GCCGTACGAGGAGAAAACTT	55.0	50.0	58.0	57.9	1196 bp	0.5	59.3