Hotspot

trnH-GUG-psbA

LSC · 62-550 · 489 bp

Back to Alocasia

Pi 0.1
MarkerSeek score 81.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
489
variable_sites
48
pi
0.1
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CAATCCACTGCCTTGATCCA AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 552-690 bp 1.0 84.0
trnH-GUG-psbA_p2 AATCCACTGCCTTGATCCAC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 551-689 bp 1.0 84.0
trnH-GUG-psbA_p3 ACAATCCACTGCCTTGATCC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 553-691 bp 1.0 84.0
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA GCTGCTGTTGAAGTTCCATC 50.0 50.0 57.9 57.4 551-689 bp 1.0 81.3
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC GCTGCTGTTGAAGTTCCATC 50.0 50.0 57.9 57.4 550-688 bp 1.0 81.3