Hotspot

rps15-ycf1

SSC · 129615-130067 · 453 bp

Back to Alnus

Pi 0.0144
MarkerSeek score 59.10
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
453
variable_sites
27
pi
0.0144
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TTGGAATTCAACGGATCCCC TCCGCGATTGAAAAGTCATT 50.0 40.0 57.9 56.1 538-553 bp 1.0 69.8
rps15-ycf1_p2 TCAACGGATCCCCTGTTTTC TCCGCGATTGAAAAGTCATT 50.0 40.0 57.8 56.1 531-546 bp 1.0 69.5
rps15-ycf1_p3 CAACGGATCCCCTGTTTTCT TCCGCGATTGAAAAGTCATT 50.0 40.0 57.8 56.1 530-545 bp 1.0 69.4
rps15-ycf1_p4 GGAATTCAACGGATCCCCTG TCCGCGATTGAAAAGTCATT 55.0 40.0 58.3 56.1 536-551 bp 1.0 68.4
rps15-ycf1_p5 TTGGAATTCAACGGATCCCC ATCCGCGATTGAAAAGTCAT 50.0 40.0 57.9 55.8 539-554 bp 0.973684 66.8