Hotspot

trnL-UAG-ccsA

SSC · 120207-120313 · 107 bp

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Pi 0.0189
MarkerSeek score 60.30
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
107
variable_sites
14
pi
0.0189
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 GTGAAATCGGTAGACACGCT TGAATCATATAGCCCTGCGC 50.0 50.0 58.0 57.9 296-305 bp 1.0 85.0
trnL-UAG-ccsA_p2 TGAAATCGGTAGACACGCTG TGAATCATATAGCCCTGCGC 50.0 50.0 58.0 57.9 295-304 bp 1.0 85.0
trnL-UAG-ccsA_p3 TAGACACGCTGCTCTTAGGA TGAATCATATAGCCCTGCGC 50.0 50.0 57.9 57.9 286-295 bp 1.0 84.3
trnL-UAG-ccsA_p4 AACAAGCCGCTATGGTGAAA TGAATCATATAGCCCTGCGC 45.0 50.0 58.1 57.9 310-319 bp 1.0 84.1
trnL-UAG-ccsA_p5 TTTGAACAAGCCGCTATGGT TGAATCATATAGCCCTGCGC 45.0 50.0 58.1 57.9 314-323 bp 1.0 84.0