Hotspot

rpl32-trnL-UAG

SSC · 118814-120126 · 1313 bp

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Pi 0.0176
MarkerSeek score 61.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1313
variable_sites
114
pi
0.0176
unique_haplotype_count
30
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGACAGACACGGTCCATACA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 1588-2037 bp 1.0 88.8
rpl32-trnL-UAG_p2 AGACAGACACGGTCCATACA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 1589-2038 bp 1.0 88.8
rpl32-trnL-UAG_p3 AGACAGACACGGTCCATACA ATAACACTTGGCGCGGTTAT 50.0 45.0 58.1 58.0 2068-2521 bp 1.0 88.4
rpl32-trnL-UAG_p4 AGACAGACACGGTCCATACA AAAAATAACACTTGGCGCGG 50.0 45.0 58.1 57.9 2072-2525 bp 1.0 88.2
rpl32-trnL-UAG_p5 AGACAGACACGGTCCATACA GCTGCATAGCCCAATACCAT 50.0 50.0 58.1 58.1 2194-2647 bp 1.0 87.8