Hotspot

ycf1-ndhF

SSC · 115443-115514 · 72 bp

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Pi 0.123
MarkerSeek score 64.40
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
SSC
length_bp
72
variable_sites
49
pi
0.123
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 ACGAAGGGGACTAAACAGGA ATATAGGGGGTGGCCGAATT 50.0 50.0 58.0 58.2 434-622 bp 1.0 89.9
ycf1-ndhF_p2 CAGAGTACCGTCACCCATTG ATATAGGGGGTGGCCGAATT 55.0 50.0 58.0 58.2 543-731 bp 1.0 89.6
ycf1-ndhF_p3 AAGAAAGCGGGGAAAGTGAG ATATAGGGGGTGGCCGAATT 50.0 50.0 58.1 58.2 490-678 bp 1.0 89.2
ycf1-ndhF_p4 ACGAAGGGGACTAAACAGGA GTTGGGGCCATAGGAATTCC 50.0 55.0 58.0 58.3 888-1076 bp 1.0 88.4
ycf1-ndhF_p5 CAGAGTACCGTCACCCATTG GTTGGGGCCATAGGAATTCC 55.0 55.0 58.0 58.3 997-1185 bp 1.0 87.8