Hotspot

ycf4-cemA

LSC · 65072-65487 · 416 bp

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Pi 0.00945
MarkerSeek score 60.50
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
416
variable_sites
20
pi
0.00945
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 ACTCCACGAGAAATTGAGCA AGAGAGAGATACACCAGGGC 45.0 55.0 57.2 57.9 555-769 bp 1.0 77.6
ycf4-cemA_p2 ACTCCACGAGAAATTGAGCA GAGAGAGATACACCAGGGCA 45.0 55.0 57.2 58.2 554-768 bp 1.0 76.5
ycf4-cemA_p3 ACTCCACGAGAAATTGAGCA AGAGAGATACACCAGGGCAA 45.0 50.0 57.2 57.7 553-767 bp 1.0 76.2
ycf4-cemA_p4 TCCCTTGACTCGTACTGATGA AGAGAGAGATACACCAGGGC 47.6 55.0 57.9 57.9 583-797 bp 1.0 75.4
ycf4-cemA_p5 GGCTATTCCCTTGACTCGTAC AGAGAGAGATACACCAGGGC 52.4 55.0 57.9 57.9 589-803 bp 1.0 75.3