Hotspot

accD-psaI

LSC · 63056-63989 · 934 bp

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Pi 0.0158
MarkerSeek score 70.60
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
934
variable_sites
70
pi
0.0158
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.96
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TCCAATCGTACCGCGTAATC AAAGAAGCCATCGCAATTGC 50.0 45.0 57.9 58.0 1000-1144 bp 1.0 88.3
accD-psaI_p2 GATCCAATCGTACCGCGTAA AAAGAAGCCATCGCAATTGC 50.0 45.0 57.9 58.0 1002-1146 bp 1.0 88.2
accD-psaI_p3 CCAATCGTACCGCGTAATCT AAAGAAGCCATCGCAATTGC 50.0 45.0 57.9 58.0 999-1143 bp 1.0 88.2
accD-psaI_p4 GATCCAATCGTACCGCGTAA TCGCAATTGCCGGAAATACT 50.0 45.0 57.9 58.3 992-1136 bp 1.0 86.3
accD-psaI_p5 TCCAATCGTACCGCGTAATC TCGCAATTGCCGGAAATACT 50.0 45.0 57.9 58.3 990-1134 bp 1.0 86.3