Hotspot

trnS-GCU-trnG-UCC

LSC · 9664-10688 · 1025 bp

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Pi 0.0253
MarkerSeek score 72.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1025
variable_sites
111
pi
0.0253
unique_haplotype_count
31
primer_available
yes
alignment_reliability
0.91
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 777-1131 bp 1.0 63.7
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 776-1130 bp 1.0 63.7
trnS-GCU-trnG-UCC_p3 ACAAAAGCGGAAAGAGAGGG ACGAATCACACTTTTACCACT 50.0 38.1 58.1 55.5 837-1191 bp 1.0 62.9
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 780-1134 bp 1.0 62.6
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 779-1133 bp 1.0 62.6