Hotspot

rps16-trnQ-UUG

LSC · 6702-8179 · 1478 bp

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Pi 0.0112
MarkerSeek score 57.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1478
variable_sites
82
pi
0.0112
unique_haplotype_count
30
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CACAGCCGACCATATCCTTC GGTATTCGGAGGTTCGACTC 55.0 55.0 58.1 57.5 1573-1628 bp 1.0 80.9
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GGTATTCGGAGGTTCGACTC 45.0 55.0 58.1 57.5 1553-1608 bp 1.0 80.9
rps16-trnQ-UUG_p3 CACAGCCGACCATATCCTTC GGGTTTTGGTCCCGGTATTC 55.0 55.0 58.1 58.5 1586-1641 bp 1.0 80.5
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.1 58.5 1566-1621 bp 1.0 80.5
rps16-trnQ-UUG_p5 CACAGCCGACCATATCCTTC TAAGGCAACGGGTTTTGGTC 55.0 50.0 58.1 58.7 1595-1650 bp 1.0 79.3