Hotspot

trnK-UUU-rps16

LSC · 4503-5541 · 1039 bp

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Pi 0.0161
MarkerSeek score 65.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1039
variable_sites
78
pi
0.0161
unique_haplotype_count
35
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TCAAAGAAGGCGGGAGTTTT 50.0 45.0 58.2 57.6 943-1179 bp 1.0 85.4
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT TTCAAAGAAGGCGGGAGTTT 50.0 45.0 58.2 57.6 944-1180 bp 1.0 85.4
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT TCAAAGAAGGCGGGAGTTTT 50.0 45.0 58.2 57.6 942-1178 bp 1.0 85.4
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTCAAAGAAGGCGGGAGTTT 50.0 45.0 58.2 57.6 943-1179 bp 1.0 85.4
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTTCAAAGAAGGCGGGAGTT 50.0 45.0 58.2 57.6 944-1180 bp 1.0 85.4