Hotspot

ycf1

SSC · 124385-130243 · 5859 bp

Back to Alniphyllum

Pi 0.0219

MarkerSeek score 57.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5859
variable_sites: 125
pi: 0.0219
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTCTGGTGGCATTACTCCTG	GCAGAATACCGTCACCCATT	55.0	50.0	58.0	58.0	1313-1415 bp	1.0	87.4
ycf1_p2	CGAATCTCATGGTCTGGTGG	GCAGAATACCGTCACCCATT	55.0	50.0	58.1	58.0	1324-1426 bp	1.0	87.3
ycf1_p3	GTCTGGTGGCATTACTCCTG	TTCCGCCCAGTTTAATAGCC	55.0	50.0	58.0	58.0	1010 bp	0.5	59.4
ycf1_p4	GTCTGGTGGCATTACTCCTG	CCGCCCAGTTTAATAGCCTT	55.0	50.0	58.0	57.9	1008 bp	0.5	59.3
ycf1_p5	CGAATCTCATGGTCTGGTGG	TTCCGCCCAGTTTAATAGCC	55.0	50.0	58.1	58.0	1021 bp	0.5	59.2