Hotspot

clpP

LSC · 69998-71423 · 1426 bp

Back to Alniphyllum

Pi 0.0218

MarkerSeek score 57.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1426
variable_sites: 31
pi: 0.0218
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGACCCTGTTAGTCCGTTCT	ATACTCCAACCACCCCATGA	50.0	50.0	58.0	58.0	2706-2712 bp	1.0	80.9
clpP_p2	TGACCCTGTTAGTCCGTTCT	TGATACTCCAACCACCCCAT	50.0	50.0	58.0	58.0	2708-2714 bp	1.0	80.9
clpP_p3	CTGAACGAGTCGCACATACA	ATACTCCAACCACCCCATGA	50.0	50.0	58.0	58.0	2417-2423 bp	1.0	80.9
clpP_p4	CTGAACGAGTCGCACATACA	TGATACTCCAACCACCCCAT	50.0	50.0	58.0	58.0	2419-2425 bp	1.0	80.9
clpP_p5	TGACCCTGTTAGTCCGTTCT	GGAATGCACCCATACTCGTT	50.0	50.0	58.0	58.0	2219-2225 bp	1.0	80.9