Hotspot

ycf1-ndhF

SSC · 110471-110836 · 366 bp

Back to Alniphyllum

Pi 0.0847

MarkerSeek score 75.90

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 366
variable_sites: 31
pi: 0.0847
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	TTCCGCCCAGTTTAATAGCC	ATATGTAGGAGGTGGTCGCA	50.0	50.0	58.0	57.9	848 bp	0.5	59.0
ycf1-ndhF_p2	TTCCGCCCAGTTTAATAGCC	TATGTAGGAGGTGGTCGCAT	50.0	50.0	58.0	57.9	847 bp	0.5	59.0
ycf1-ndhF_p3	CCGCCCAGTTTAATAGCCTT	ATATGTAGGAGGTGGTCGCA	50.0	50.0	57.9	57.9	846 bp	0.5	58.9
ycf1-ndhF_p4	CCGCCCAGTTTAATAGCCTT	TATGTAGGAGGTGGTCGCAT	50.0	50.0	57.9	57.9	845 bp	0.5	58.9
ycf1-ndhF_p5	ACGTTTCCGCCCAGTTTAAT	TATGTAGGAGGTGGTCGCAT	45.0	50.0	58.1	57.9	851 bp	0.5	58.5