Hotspot

rpl14-rpl16

LSC · 80563-80686 · 124 bp

Back to Alniphyllum

Pi 0.0161

MarkerSeek score 61.80

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 2
pi: 0.0161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	GAGCATATCGGCGATTGCTA	CAATAGCGGCGTCCAAAATG	50.0	50.0	58.0	58.2	277-278 bp	1.0	79.2
rpl14-rpl16_p2	GAGCATATCGGCGATTGCTA	ATTTCAATAGCGGCGTCCAA	50.0	45.0	58.0	58.3	281-282 bp	1.0	78.5
rpl14-rpl16_p3	GAGCATATCGGCGATTGCTA	TCAATAGCGGCGTCCAAAAT	50.0	45.0	58.0	58.3	278-279 bp	1.0	78.5
rpl14-rpl16_p4	TCGGCGATTGCTATCTCCTA	CAATAGCGGCGTCCAAAATG	50.0	50.0	58.1	58.2	270-271 bp	1.0	78.4
rpl14-rpl16_p5	CCGCTGTTATCCGCTACATT	CAATAGCGGCGTCCAAAATG	50.0	50.0	58.1	58.2	221-222 bp	1.0	78.3