Hotspot

rpl20-rps12

LSC · 68941-69715 · 775 bp

Back to Alniphyllum

Pi 0.00517

MarkerSeek score 61.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 775
variable_sites: 4
pi: 0.00517
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	AATGAGCACCTCGAAAGCTT	AATGTCACGAAATCTCCCGC	45.0	50.0	57.8	58.6	925-926 bp	1.0	73.9
rpl20-rps12_p2	TCCCCGTCTAATTCTGGTCA	AATGTCACGAAATCTCCCGC	50.0	50.0	57.8	58.6	861-862 bp	1.0	73.5
rpl20-rps12_p3	GAATGAGCACCTCGAAAGCT	AATGTCACGAAATCTCCCGC	50.0	50.0	58.3	58.6	926-927 bp	1.0	73.2
rpl20-rps12_p4	GAGCACCTCGAAAGCTTGAT	AATGTCACGAAATCTCCCGC	50.0	50.0	58.3	58.6	922-923 bp	1.0	73.2
rpl20-rps12_p5	AATGAGCACCTCGAAAGCTT	CACGAAATCTCCCGCTCTTC	45.0	55.0	57.8	58.7	920-921 bp	1.0	73.2