Hotspot

rbcL-accD

LSC · 55510-57273 · 1764 bp

Back to Alniphyllum

Pi 0.0288

MarkerSeek score 75.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1764
variable_sites: 42
pi: 0.0288
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AATGAGGGACGTGATCTTGC	TACCAAAACTGGCTGTCACC	50.0	50.0	58.0	58.0	2018-2329 bp	1.0	85.1
rbcL-accD_p2	GAGGCTAGCAAATGGAGTCC	TACCAAAACTGGCTGTCACC	55.0	50.0	58.0	58.0	1973-2284 bp	1.0	85.1
rbcL-accD_p3	GCTCTGACCGAGATCTTTGG	TACCAAAACTGGCTGTCACC	55.0	50.0	58.1	58.0	2147-2458 bp	1.0	84.7
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	TACCAAAACTGGCTGTCACC	50.0	50.0	57.9	58.0	1943-2254 bp	1.0	84.5
rbcL-accD_p5	ACCGAGATCTTTGGGGATGA	TACCAAAACTGGCTGTCACC	50.0	50.0	58.1	58.0	2141-2452 bp	1.0	84.3