Hotspot

trnS-GCU-psbI

LSC · 25571-25710 · 140 bp

Back to Alniphyllum

Pi 0.0144

MarkerSeek score 61.50

Primer available yes

Label trnS-GCU-psbI

Feature Metadata

label_name: trnS-GCU-psbI
feature_type: igs
region: LSC
length_bp: 140
variable_sites: 2
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbI_p1	TAAAGCGGCGGATTGCTAAT	CGTAATCCTGGACGTGAAGA	45.0	50.0	58.0	57.1	231-232 bp	1.0	73.3
trnS-GCU-psbI_p2	TTTCAATTGGGGGAGATGGC	CGTAATCCTGGACGTGAAGA	50.0	50.0	58.1	57.1	260-261 bp	1.0	72.4
trnS-GCU-psbI_p3	GGAGATGGCTGAGTGGACTA	CGTAATCCTGGACGTGAAGA	55.0	50.0	58.2	57.1	249-250 bp	1.0	71.7
trnS-GCU-psbI_p4	TAAAGCGGCGGATTGCTAAT	CGTAATCCTGGACGTGAAGAA	45.0	47.6	58.0	57.8	231-232 bp	1.0	70.9
trnS-GCU-psbI_p5	ATGGCTGAGTGGACTAAAGC	CGTAATCCTGGACGTGAAGA	50.0	50.0	57.6	57.1	245-246 bp	1.0	70.2