Hotspot

trnR-UCU-trnG-GCC

LSC · 23871-24125 · 255 bp

Back to Alniphyllum

Pi 0.0588

MarkerSeek score 79.70

Primer available yes

Label trnR-UCU-trnG-GCC

Feature Metadata

label_name: trnR-UCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 255
variable_sites: 15
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-GCC_p1	AGAAGACCTCTGTCCTATCCA	AGCCTTCCAAGCTAACGATG	47.6	50.0	56.9	58.0	335-337 bp	1.0	66.9
trnR-UCU-trnG-GCC_p2	AGAAGACCTCTGTCCTATCCA	CCTAGCCTTCCAAGCTAACG	47.6	55.0	56.9	58.1	338-340 bp	1.0	66.7
trnR-UCU-trnG-GCC_p3	AGGTTTAGAAGACCTCTGTCCT	AGCCTTCCAAGCTAACGATG	45.5	50.0	57.9	58.0	341-343 bp	1.0	66.2
trnR-UCU-trnG-GCC_p4	AGGTTTAGAAGACCTCTGTCCT	CCTAGCCTTCCAAGCTAACG	45.5	55.0	57.9	58.1	344-346 bp	1.0	65.9
trnR-UCU-trnG-GCC_p5	AGAAGACCTCTGTCCTATCCA	CCCTAGCCTTCCAAGCTAAC	47.6	55.0	56.9	57.7	339-341 bp	1.0	64.4