primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-GCC_p1	trnR-UCU-trnG-GCC	1	AGAAGACCTCTGTCCTATCCA	AGCCTTCCAAGCTAACGATG	21	20	47.6	50.0	56.9	58.0	0.0	0.0	2.081443	23871	24125	335-337	1.000000	66.9
trnR-UCU-trnG-GCC_p2	trnR-UCU-trnG-GCC	2	AGAAGACCTCTGTCCTATCCA	CCTAGCCTTCCAAGCTAACG	21	20	47.6	55.0	56.9	58.1	0.0	0.0	2.110280	23871	24125	338-340	1.000000	66.7
trnR-UCU-trnG-GCC_p3	trnR-UCU-trnG-GCC	3	AGGTTTAGAAGACCTCTGTCCT	AGCCTTCCAAGCTAACGATG	22	20	45.5	50.0	57.9	58.0	0.0	0.0	2.164771	23871	24125	341-343	1.000000	66.2
trnR-UCU-trnG-GCC_p4	trnR-UCU-trnG-GCC	4	AGGTTTAGAAGACCTCTGTCCT	CCTAGCCTTCCAAGCTAACG	22	20	45.5	55.0	57.9	58.1	0.0	0.0	2.193608	23871	24125	344-346	1.000000	65.9
trnR-UCU-trnG-GCC_p5	trnR-UCU-trnG-GCC	5	AGAAGACCTCTGTCCTATCCA	CCCTAGCCTTCCAAGCTAAC	21	20	47.6	55.0	56.9	57.7	0.0	0.0	2.385293	23871	24125	339-341	1.000000	64.4
trnG-GCC_p1	trnG-GCC	1	CGAACCTCTTACGGTGGAAG	AGGAAACGGAAAGAGAGGGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.033466	24126	24892	2072-2138	1.000000	83.0
trnG-GCC_p2	trnG-GCC	2	CGAACCTCTTACGGTGGAAG	ATTAGCAATCCGCCGCTTTA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.046037	24126	24892	2018-2084	1.000000	82.9
trnG-GCC_p3	trnG-GCC	3	CGCAGAAGCCCTTTTGAAAG	AGGAAACGGAAAGAGAGGGA	20	20	50.0	50.0	57.9	58.0	3.1	0.0	0.081730	24126	24892	1895-1961	1.000000	82.9
trnG-GCC_p4	trnG-GCC	4	CCAATCCGAACCTCTTACGG	AGGAAACGGAAAGAGAGGGA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.089703	24126	24892	2078-2144	1.000000	82.5
trnG-GCC_p5	trnG-GCC	5	CCCAATCCGAACCTCTTACG	AGGAAACGGAAAGAGAGGGA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.089703	24126	24892	2079-2145	1.000000	82.5
trnS-GCU-psbI_p1	trnS-GCU-psbI	1	TAAAGCGGCGGATTGCTAAT	CGTAATCCTGGACGTGAAGA	20	20	45.0	50.0	58.0	57.1	9.8	0.0	0.971702	25571	25710	231-232	1.000000	73.3
trnS-GCU-psbI_p2	trnS-GCU-psbI	2	TTTCAATTGGGGGAGATGGC	CGTAATCCTGGACGTGAAGA	20	20	50.0	50.0	58.1	57.1	5.4	0.0	1.068826	25571	25710	260-261	1.000000	72.4
trnS-GCU-psbI_p3	trnS-GCU-psbI	3	GGAGATGGCTGAGTGGACTA	CGTAATCCTGGACGTGAAGA	20	20	55.0	50.0	58.2	57.1	0.0	0.0	1.151709	25571	25710	249-250	1.000000	71.7
trnS-GCU-psbI_p4	trnS-GCU-psbI	4	TAAAGCGGCGGATTGCTAAT	CGTAATCCTGGACGTGAAGAA	20	21	45.0	47.6	58.0	57.8	9.8	0.0	1.264552	25571	25710	231-232	1.000000	70.9
trnS-GCU-psbI_p5	trnS-GCU-psbI	5	ATGGCTGAGTGGACTAAAGC	CGTAATCCTGGACGTGAAGA	20	20	50.0	50.0	57.6	57.1	5.7	0.0	1.343443	25571	25710	245-246	1.000000	70.2
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	21	20	47.6	50.0	58.0	58.1	8.2	0.0	1.134847	42700	43544	934-934	1.000000	71.3
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	58.0	58.2	8.2	0.0	1.191833	42700	43544	955-955	1.000000	70.9
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	21	20	47.6	45.0	58.0	57.8	8.2	5.6	1.233148	42700	43544	905-905	1.000000	70.6
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	21	20	47.6	50.0	58.0	58.4	8.2	0.0	1.427808	42700	43544	935-935	1.000000	69.0
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTTATCCGCGATCTAGGCAT	CCCTCGGTAAACAAAAGCCT	20	20	45.0	50.0	56.6	58.1	8.2	0.0	1.514239	42700	43544	932-932	1.000000	68.3
rbcL-accD_p1	rbcL-accD	1	AATGAGGGACGTGATCTTGC	TACCAAAACTGGCTGTCACC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054179	55510	57273	2018-2329	1.000000	85.1
rbcL-accD_p2	rbcL-accD	2	GAGGCTAGCAAATGGAGTCC	TACCAAAACTGGCTGTCACC	20	20	55.0	50.0	58.0	58.0	18.2	0.0	0.063924	55510	57273	1973-2284	1.000000	85.1
rbcL-accD_p3	rbcL-accD	3	GCTCTGACCGAGATCTTTGG	TACCAAAACTGGCTGTCACC	20	20	55.0	50.0	58.1	58.0	12.2	0.0	0.091094	55510	57273	2147-2458	1.000000	84.7
rbcL-accD_p4	rbcL-accD	4	GCTGCTTGTGAGGTATGGAA	TACCAAAACTGGCTGTCACC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.138201	55510	57273	1943-2254	1.000000	84.5
rbcL-accD_p5	rbcL-accD	5	ACCGAGATCTTTGGGGATGA	TACCAAAACTGGCTGTCACC	20	20	50.0	50.0	58.1	58.0	16.7	0.0	0.144765	55510	57273	2141-2452	1.000000	84.3
accD-psaI_p1	accD-psaI	1	GAGTTATTTCAGCTCCACGC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	56.9	58.0	0.0	26.9	1.122618	57937	58718	887-906	1.000000	73.6
accD-psaI_p2	accD-psaI	2	GAGTTATTTCAGCTCCACGC	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	56.9	57.9	0.0	26.8	1.199152	57937	58718	893-912	1.000000	73.0
accD-psaI_p3	accD-psaI	3	GAGTTATTTCAGCTCCACGC	GCCGGAAATACTAGGCCTAC	20	20	50.0	55.0	56.9	57.5	0.0	8.9	1.547910	57937	58718	876-895	1.000000	70.2
accD-psaI_p4	accD-psaI	4	CGCTTTCGTTCCTTTGAATCA	CCATTGCAATTGCCGGAAAT	21	20	42.9	45.0	57.5	58.0	0.0	26.9	1.563161	57937	58718	870-889	1.000000	70.1
accD-psaI_p5	accD-psaI	5	CGCTTTCGTTCCTTTGAATCA	AAGAAGCCATTGCAATTGCC	21	20	42.9	45.0	57.5	57.9	0.0	26.8	1.639695	57937	58718	876-895	1.000000	69.5
petA-psbJ_p1	petA-psbJ	1	CCATTACGTGTTCAAGGCCT	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.2	57.6	0.0	4.1	0.543756	62647	63677	1171-1174	1.000000	76.3
petA-psbJ_p2	petA-psbJ	2	TCCATTACGTGTTCAAGGCC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.2	57.6	0.0	4.1	0.548898	62647	63677	1172-1175	1.000000	76.2
petA-psbJ_p3	petA-psbJ	3	ACGTGTTCAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.5	57.6	17.6	4.1	0.894921	62647	63677	1166-1169	1.000000	73.5
petA-psbJ_p4	petA-psbJ	4	CGTGTTCAAGGCCTTTTGTT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.4	57.6	17.6	4.1	0.942422	62647	63677	1165-1168	1.000000	73.1
petA-psbJ_p5	petA-psbJ	5	CCATTACGTGTTCAAGGCCT	TGGTTCATATTCCGGATTGGG	20	21	50.0	47.6	58.2	57.8	0.0	4.1	1.400956	62647	63677	1179-1182	1.000000	69.4
rpl20-rps12_p1	rpl20-rps12	1	AATGAGCACCTCGAAAGCTT	AATGTCACGAAATCTCCCGC	20	20	45.0	50.0	57.8	58.6	7.3	0.0	0.821503	68941	69715	925-926	1.000000	73.9
rpl20-rps12_p2	rpl20-rps12	2	TCCCCGTCTAATTCTGGTCA	AATGTCACGAAATCTCCCGC	20	20	50.0	50.0	57.8	58.6	0.0	0.0	0.871656	68941	69715	861-862	1.000000	73.5
rpl20-rps12_p3	rpl20-rps12	3	GAATGAGCACCTCGAAAGCT	AATGTCACGAAATCTCCCGC	20	20	50.0	50.0	58.3	58.6	5.7	0.0	0.898655	68941	69715	926-927	1.000000	73.2
rpl20-rps12_p4	rpl20-rps12	4	GAGCACCTCGAAAGCTTGAT	AATGTCACGAAATCTCCCGC	20	20	50.0	50.0	58.3	58.6	0.0	0.0	0.898655	68941	69715	922-923	1.000000	73.2
rpl20-rps12_p5	rpl20-rps12	5	AATGAGCACCTCGAAAGCTT	CACGAAATCTCCCGCTCTTC	20	20	45.0	55.0	57.8	58.7	7.3	0.0	0.909229	68941	69715	920-921	1.000000	73.2
clpP_p1	clpP	1	TGACCCTGTTAGTCCGTTCT	ATACTCCAACCACCCCATGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.025561	69998	71423	2706-2712	1.000000	80.9
clpP_p2	clpP	2	TGACCCTGTTAGTCCGTTCT	TGATACTCCAACCACCCCAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.025561	69998	71423	2708-2714	1.000000	80.9
clpP_p3	clpP	3	CTGAACGAGTCGCACATACA	ATACTCCAACCACCCCATGA	20	20	50.0	50.0	58.0	58.0	13.6	0.0	0.043002	69998	71423	2417-2423	1.000000	80.9
clpP_p4	clpP	4	CTGAACGAGTCGCACATACA	TGATACTCCAACCACCCCAT	20	20	50.0	50.0	58.0	58.0	13.6	0.0	0.043002	69998	71423	2419-2425	1.000000	80.9
clpP_p5	clpP	5	TGACCCTGTTAGTCCGTTCT	GGAATGCACCCATACTCGTT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.043757	69998	71423	2219-2225	1.000000	80.9
psbT-psbN_p1	psbT-psbN	1	CGAGAACCACCTAAAGTTCCA	TGTATACCGCTTTTGGGCAA	21	20	47.6	45.0	57.6	57.8	0.0	0.0	1.598181	73699	73762	168-168	1.000000	67.7
psbT-psbN_p2	psbT-psbN	2	CGAGAACCACCTAAAGTTCCA	TTGTATACCGCTTTTGGGCA	21	20	47.6	45.0	57.6	57.8	0.0	0.0	1.598181	73699	73762	169-169	1.000000	67.7
psbT-psbN_p3	psbT-psbN	3	CGAGAACCACCTAAAGTTCCA	TCGAGGAACATGGGGACTAA	21	20	47.6	50.0	57.6	57.8	0.0	0.0	1.637541	73699	73762	123-123	1.000000	67.6
psbT-psbN_p4	psbT-psbN	4	CGAGAACCACCTAAAGTTCCA	TTCGAGGAACATGGGGACTA	21	20	47.6	50.0	57.6	57.8	0.0	0.0	1.637541	73699	73762	124-124	1.000000	67.5
psbT-psbN_p5	psbT-psbN	5	CGAGAACCACCTAAAGTTCCA	CATTCGAGGAACATGGGGAC	21	20	47.6	55.0	57.6	58.3	0.0	0.0	1.727841	73699	73762	126-126	1.000000	66.8
rpl14-rpl16_p1	rpl14-rpl16	1	GAGCATATCGGCGATTGCTA	CAATAGCGGCGTCCAAAATG	20	20	50.0	50.0	58.0	58.2	36.0	9.4	0.177381	80563	80686	277-278	1.000000	79.2
rpl14-rpl16_p2	rpl14-rpl16	2	GAGCATATCGGCGATTGCTA	ATTTCAATAGCGGCGTCCAA	20	20	50.0	45.0	58.0	58.3	36.0	9.4	0.258476	80563	80686	281-282	1.000000	78.5
rpl14-rpl16_p3	rpl14-rpl16	3	GAGCATATCGGCGATTGCTA	TCAATAGCGGCGTCCAAAAT	20	20	50.0	45.0	58.0	58.3	36.0	9.4	0.258476	80563	80686	278-279	1.000000	78.5
rpl14-rpl16_p4	rpl14-rpl16	4	TCGGCGATTGCTATCTCCTA	CAATAGCGGCGTCCAAAATG	20	20	50.0	50.0	58.1	58.2	5.0	9.4	0.268036	80563	80686	270-271	1.000000	78.4
rpl14-rpl16_p5	rpl14-rpl16	5	CCGCTGTTATCCGCTACATT	CAATAGCGGCGTCCAAAATG	20	20	50.0	50.0	58.1	58.2	0.0	9.4	0.307808	80563	80686	221-222	1.000000	78.3
ycf15-trnL-CAA_p1	ycf15-trnL-CAA	1	TCTTTCGGATAGGCCCTGAA	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.1	58.0	0.0	15.9	0.133701	93228	93596	500-500	1.000000	79.7
ycf15-trnL-CAA_p2	ycf15-trnL-CAA	2	TCTTTCGGATAGGCCCTGAA	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	58.1	58.0	0.0	15.9	0.135071	93228	93596	502-502	1.000000	79.7
ycf15-trnL-CAA_p3	ycf15-trnL-CAA	3	TTCTCTTTCGGATAGGCCCT	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	57.8	58.0	0.0	15.9	0.184078	93228	93596	503-503	1.000000	79.3
ycf15-trnL-CAA_p4	ycf15-trnL-CAA	4	TTCTCTTTCGGATAGGCCCT	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	57.8	58.0	0.0	15.9	0.185448	93228	93596	505-505	1.000000	79.3
ycf15-trnL-CAA_p5	ycf15-trnL-CAA	5	TCTTTCGGATAGGCCCTGAA	GCCTTGGTGGTGAAATGGTA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.207998	93228	93596	512-512	1.000000	79.1
ycf1-ndhF_p1	ycf1-ndhF	1	TTCCGCCCAGTTTAATAGCC	ATATGTAGGAGGTGGTCGCA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.129142	110471	110836	848-848	0.500000	59.0
ycf1-ndhF_p2	ycf1-ndhF	2	TTCCGCCCAGTTTAATAGCC	TATGTAGGAGGTGGTCGCAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.129142	110471	110836	847-847	0.500000	59.0
ycf1-ndhF_p3	ycf1-ndhF	3	CCGCCCAGTTTAATAGCCTT	ATATGTAGGAGGTGGTCGCA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.134922	110471	110836	846-846	0.500000	58.9
ycf1-ndhF_p4	ycf1-ndhF	4	CCGCCCAGTTTAATAGCCTT	TATGTAGGAGGTGGTCGCAT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.134922	110471	110836	845-845	0.500000	58.9
ycf1-ndhF_p5	ycf1-ndhF	5	ACGTTTCCGCCCAGTTTAAT	TATGTAGGAGGTGGTCGCAT	20	20	45.0	50.0	58.1	57.9	0.0	0.0	0.181895	110471	110836	851-851	0.500000	58.5
ycf1_p1	ycf1	1	GTCTGGTGGCATTACTCCTG	GCAGAATACCGTCACCCATT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.068225	128620	128739	1313-1415	1.000000	87.4
ycf1_p2	ycf1	2	CGAATCTCATGGTCTGGTGG	GCAGAATACCGTCACCCATT	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.092999	128620	128739	1324-1426	1.000000	87.3
ycf1_p3	ycf1	3	GTCTGGTGGCATTACTCCTG	TTCCGCCCAGTTTAATAGCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.076840	128620	128739	1010-1010	0.500000	59.4
ycf1_p4	ycf1	4	GTCTGGTGGCATTACTCCTG	CCGCCCAGTTTAATAGCCTT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.082621	128620	128739	1008-1008	0.500000	59.3
ycf1_p5	ycf1	5	CGAATCTCATGGTCTGGTGG	TTCCGCCCAGTTTAATAGCC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.101614	128620	128739	1021-1021	0.500000	59.2
trnL-CAA-ycf15_p1	trnL-CAA-ycf15	1	AAATGGTAGACACGCGAGAC	TCTTTCGGATAGGCCCTGAA	20	20	50.0	50.0	58.0	58.1	15.9	0.0	0.133701	145499	145867	500-500	1.000000	79.7
trnL-CAA-ycf15_p2	trnL-CAA-ycf15	2	TGAAATGGTAGACACGCGAG	TCTTTCGGATAGGCCCTGAA	20	20	50.0	50.0	58.0	58.1	15.9	0.0	0.135071	145499	145867	502-502	1.000000	79.7
trnL-CAA-ycf15_p3	trnL-CAA-ycf15	3	AAATGGTAGACACGCGAGAC	TTCTCTTTCGGATAGGCCCT	20	20	50.0	50.0	58.0	57.8	15.9	0.0	0.184078	145499	145867	503-503	1.000000	79.3
trnL-CAA-ycf15_p4	trnL-CAA-ycf15	4	TGAAATGGTAGACACGCGAG	TTCTCTTTCGGATAGGCCCT	20	20	50.0	50.0	58.0	57.8	15.9	0.0	0.185448	145499	145867	505-505	1.000000	79.3
trnL-CAA-ycf15_p5	trnL-CAA-ycf15	5	GCCTTGGTGGTGAAATGGTA	TCTTTCGGATAGGCCCTGAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.207998	145499	145867	512-512	1.000000	79.1