Hotspot

petL-petG

LSC · 69718-69875 · 158 bp

Back to Alniaria

Pi 0.00422

MarkerSeek score 34.10

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 158
variable_sites: 1
pi: 0.00422
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	CGGTCTGAGCAAGATACGAC	CAACTGATCACCACGTCTGT	55.0	50.0	58.2	57.8	291 bp	1.0	77.7
petL-petG_p2	TCGGTCTGAGCAAGATACGA	CAACTGATCACCACGTCTGT	50.0	50.0	58.3	57.8	292 bp	1.0	77.0
petL-petG_p3	CGGTCTGAGCAAGATACGAC	ATCCAACTGATCACCACGTC	55.0	50.0	58.2	57.6	294 bp	1.0	75.8
petL-petG_p4	TCGGTCTGAGCAAGATACGA	ATCCAACTGATCACCACGTC	50.0	50.0	58.3	57.6	295 bp	1.0	75.1
petL-petG_p5	CGGTCTGAGCAAGATACGAC	CCAACTGATCACCACGTCTG	55.0	55.0	58.2	58.6	292 bp	1.0	74.4