Hotspot

rps4-trnT-UGU

LSC · 49294-49673 · 380 bp

Back to Alniaria

Pi 0.0105

MarkerSeek score 46.10

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 380
variable_sites: 6
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	540-548 bp	1.0	79.8
rps4-trnT-UGU_p2	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	544-552 bp	1.0	79.4
rps4-trnT-UGU_p3	CTTCCGGCTCTAGGCTTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	532-540 bp	1.0	79.1
rps4-trnT-UGU_p4	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	508-516 bp	1.0	79.0
rps4-trnT-UGU_p5	TTTCTAAGATCGCTTCCGGC	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	512-520 bp	1.0	78.6